Microfluidics applications for high-throughput single cell sequencing

dc.contributor.authorZhou, Wen-min
dc.contributor.authorYan, Yan-yan
dc.contributor.authorGuo, Qiao-ru
dc.contributor.authorJi, Hong
dc.contributor.authorWang, Hui
dc.contributor.authorXu, Tian-tian
dc.contributor.authorMakabel, Bolat
dc.contributor.authorPilarsky, Christian
dc.contributor.authorHe, Gen
dc.contributor.authorYu, Xi-yong
dc.contributor.authorZhang, Jian-ye
dc.date.accessioned2021-11-26
dc.date.available2021-10-11
dc.date.created2021
dc.date.issued2021-11-26
dc.description.abstractThe inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, which is critical for disease diagnosis and treatment. Substantial evidences show that the majority of traditional gene profiling methods mask the difference of individual cells. Single cell sequencing can provide data to characterize the inherent heterogeneity of individual cells, and reveal complex and rare cell populations. Different microfluidic technologies have emerged for single cell researches and become the frontiers and hot topics over the past decade. In this review article, we introduce the processes of single cell sequencing, and review the principles of microfluidics for single cell analysis. Also, we discuss the common high-throughput single cell sequencing technologies along with their advantages and disadvantages. Lastly, microfluidics applications in single cell sequencing technology for the diagnosis of cancers and immune system diseases are briefly illustrated.en
dc.identifier.citationJournal of Nanobiotechnology 19 (2021): 312. <https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-021-01045-6>
dc.identifier.doihttps://doi.org/10.1186/s12951-021-01045-6
dc.identifier.opus-id17699
dc.identifier.urihttps://open.fau.de/handle/openfau/17699
dc.identifier.urnurn:nbn:de:bvb:29-opus4-176998
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjectSingle cell separation
dc.subjectSingle cell RNA sequencing (scRNA-seq)
dc.subjectHigh-throughput
dc.subjectMicrofluidic
dc.subjectDroplets
dc.subjectBiomedical applications
dc.subject.ddcDDC Classification::6 Technik, Medizin, angewandte Wissenschaften :: 61 Medizin und Gesundheit :: 610 Medizin und Gesundheit
dc.titleMicrofluidics applications for high-throughput single cell sequencingen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.document.articlenumber312
local.journal.titleJournal of Nanobiotechnology
local.journal.volume19
local.sendToDnbfree*
local.subject.fakultaetMedizinische Fakultät
local.subject.sammlungUniversität Erlangen-Nürnberg / Open Access Artikel ohne Förderung / Open Access Artikel ohne Förderung 2021
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