Effect of Citrate- and Gold-Stabilized Superparamagnetic Iron Oxide Nanoparticles on Head and Neck Tumor Cell Lines during Combination Therapy with Ionizing Radiation

dc.contributor.authorSchreiber, Christoph
dc.contributor.authorFranzen, Tim
dc.contributor.authorHildebrand, Laura
dc.contributor.authorStein, René
dc.contributor.authorFriedrich, Bernhard
dc.contributor.authorTietze, Rainer
dc.contributor.authorFietkau, Rainer
dc.contributor.authorDistel, Luitpold V.
dc.date.accessioned2023-06-26
dc.date.available2023-10-09T22:30:01Z
dc.date.created2022
dc.date.issued2023-06-26
dc.description.abstractHead and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide. They are associated with alcohol and tobacco consumption, as well as infection with human papillomaviruses (HPV). Therapeutic options include radiochemotherapy, surgery or chemotherapy. Nanoparticles are becoming more and more important in medicine. They can be used diagnostically, but also therapeutically. In order to provide therapeutic alternatives in the treatment of HNSCC, the effect of citrate-coated superparamagnetic iron oxide nanoparticles (Citrate-SPIONs) and gold-coated superparamagnetic iron oxide nanoparticles (Au-SPIONs) in combination with ionizing irradiation (IR) on two HPV positive and two HPV negative HNSCC and healthy fibroblasts and keratinocytes cell lines were tested. Effects on apoptosis and necrosis were analyzed by using flow cytometry. Cell survival studies were performed with a colony formation assay. To better understand where the SPIONs interact, light microscopy images and immunofluorescence studies were performed. The HNSCC and healthy cell lines showed different responses to the investigated SPIONs. The cytotoxic effects of SPIONs, in combination with IR, are dependent on the type of SPIONs, the dose administered and the cell type treated. They are independent of HPV status. Reasons for the different cytotoxic effect are probably the different compositions of the SPIONs and the related different interaction of the SPIONs intracellularly and paramembranously, which lead to different strong formations of double strand breaks.en
dc.identifier.citationBioengineering 9.12 (2022): 806. <https://www.mdpi.com/2306-5354/9/12/806>
dc.identifier.doihttps://doi.org/10.3390/bioengineering9120806
dc.identifier.issn2306-5354
dc.identifier.opus-id23398
dc.identifier.urihttps://open.fau.de/handle/openfau/23398
dc.identifier.urnurn:nbn:de:bvb:29-opus4-233983
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjecthead and neck cancer cell lines
dc.subjectnanoparticles
dc.subjectsuperparamagnetic iron oxide nanoparticles
dc.subjectionizing radiation
dc.subjectinteractions
dc.subjectcitrate
dc.subjectgold
dc.subject.ddcDDC Classification::6 Technik, Medizin, angewandte Wissenschaften :: 61 Medizin und Gesundheit :: 610 Medizin und Gesundheit
dc.titleEffect of Citrate- and Gold-Stabilized Superparamagnetic Iron Oxide Nanoparticles on Head and Neck Tumor Cell Lines during Combination Therapy with Ionizing Radiationen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.document.articlenumber806
local.journal.issue12
local.journal.titleBioengineering
local.journal.volume9
local.sendToDnbfree*
local.subject.fakultaetMedizinische Fakultät
local.subject.sammlungUniversität Erlangen-Nürnberg / Von der FAU geförderte Open Access Artikel / Von der FAU geförderte Open Access Artikel 2022
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