In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

Language
en
Document Type
Article
Issue Date
2019-08-21
First published
2017-07-03
Issue Year
2017
Authors
Haro Durand, Luis
Vargas, Gabriela
Vera-Mesones, Rosa
Baldi, Alberto
Zago, María
Fanovich, María
Boccaccini, Aldo
Gorustovich, Alejandro
Editor
Publisher
MDPI
Abstract

Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.

Journal Title
Materials
Volume
10
Issue
7
Citation
Materials 10.7 (2017). <https://www.mdpi.com/1996-1944/10/7/740>
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