Targeting c-MYC with T-Cells

Language
en
Document Type
Article
Issue Date
2013-10-16
Issue Year
2013
Authors
Helm, Florian
Kammertoens, Thomas
Lehmann, Frank M.
Wilke, Andrea
Bruns, Heiko
Mautner, Josef
Bornkamm, Georg W.
Gerbitz, Armin
Editor
Abstract

Over-expression of the proto-oncogene c-MYC is frequently observed in a variety of tumors and is a hallmark of Burkitt´s lymphoma. The fact that many tumors are oncogene-addicted to c-MYC, renders c-MYC a powerful target for anti-tumor therapy. Using a xenogenic vaccination strategy by immunizing C57BL/6 mice with human c-MYC protein or non-homologous peptides, we show that the human c-MYC protein, despite its high homology between mouse and man, contains several immunogenic epitopes presented in the context of murine H2b haplotype. We identified an MHC class II-restricted CD4+ T-cell epitope and therein an MHC class I-restricted CD8+ T-cell epitope (SSPQGSPEPL) that, after prime/boost immunization, protected up to 25% of mice against a lethal lymphoma challenge. Lymphoma-rejecting animals contained MHC multimer-binding CD8+ cell within the peripheral blood and displayed in vivo cytolytic activity with specificity for SSPQGSPEPL. Taken together these data suggest that oncogenic c-MYC can be targeted with specific T-cells.

Journal Title
PLoS ONE
Volume
8
Issue
10
Citation
PLoS ONE 8.10 (2013): 15.10.2013 <http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0077375>
DOI
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