Consecutive-Day Ventricular and Atrial Cardiomyocyte Isolations from the Same Heart: Shifting the Cost–Benefit Balance of Cardiac Primary Cell Research

dc.contributor.authorGreiner, Joachim
dc.contributor.authorSchiatti, Teresa
dc.contributor.authorKaltenbacher, Wenzel
dc.contributor.authorDente, Marica
dc.contributor.authorSemenjakin, Alina
dc.contributor.authorKok, Thomas
dc.contributor.authorFiegle, Dominik J.
dc.contributor.authorSeidel, Thomas
dc.contributor.authorRavens, Ursula
dc.contributor.authorKohl, Peter
dc.contributor.authorPeyronnet, Rémi
dc.contributor.authorRog-Zielinska, Eva A.
dc.date.accessioned2022-02-10
dc.date.available2023-10-09T18:07:26Z
dc.date.created2022
dc.date.issued2022-02-10
dc.description.abstractFreshly isolated primary cardiomyocytes (CM) are indispensable for cardiac research. Experimental CM research is generally incompatible with life of the donor animal, while human heart samples are usually small and scarce. CM isolation from animal hearts, traditionally performed by coronary artery perfusion of enzymes, liberates millions of cells from the heart. However, due to progressive cell remodeling following isolation, freshly isolated primary CM need to be used within 4–8 h post-isolation for most functional assays, meaning that the majority of cells is essentially wasted. In addition, coronary perfusion-based isolation cannot easily be applied to human tissue biopsies, and it does not straightforwardly allow for assessment of regional differences in CM function within the same heart. Here, we provide a method of multi-day CM isolation from one animal heart, yielding calcium-tolerant ventricular and atrial CM. This is based on cell isolation from cardiac tissue slices following repeated (usually overnight) storage of the tissue under conditions that prolong CM viability beyond the day of organ excision by two additional days. The maintenance of cells in their near-native microenvironment slows the otherwise rapid structural and functional decline seen in isolated CM during attempts for prolonged storage or culture. Multi-day slice-based CM isolation increases the amount of useful information gained per animal heart, improving reproducibility and reducing the number of experimental animals required in basic cardiac research. It also opens the doors to novel experimental designs, including exploring same-heart regional differences.en
dc.identifier.citationCells 11.2 (2022): 233. <https://www.mdpi.com/2073-4409/11/2/233>
dc.identifier.doihttps://doi.org/10.3390/cells11020233
dc.identifier.issn2073-4409
dc.identifier.opus-id18343
dc.identifier.urihttps://open.fau.de/handle/openfau/18343
dc.identifier.urnurn:nbn:de:bvb:29-opus4-183435
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjectcardiomyocyte isolation
dc.subjectsingle cell
dc.subjectsarcomere shortening
dc.subjecttissue preservation
dc.subject.ddcDDC Classification::6 Technik, Medizin, angewandte Wissenschaften :: 61 Medizin und Gesundheit :: 610 Medizin und Gesundheit
dc.titleConsecutive-Day Ventricular and Atrial Cardiomyocyte Isolations from the Same Heart: Shifting the Cost–Benefit Balance of Cardiac Primary Cell Researchen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.date.prevpublished2022-01-11
local.document.articlenumber233
local.journal.issue2
local.journal.titleCells
local.journal.volume11
local.sendToDnbfree*
local.subject.fakultaetMedizinische Fakultät
local.subject.importimport
local.subject.sammlungUniversität Erlangen-Nürnberg / Eingespielte Open Access Artikel / Eingespielte Open Access Artikel 2022
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