Generalized resistance to pruritogen-induced scratching in the C3H/HeJ strain

dc.contributor.authorZhang, Yanbin
dc.contributor.authorRichter, Nicole
dc.contributor.authorKönig, Christine
dc.contributor.authorKremer, Andreas E.
dc.contributor.authorZimmermann, Katharina
dc.date.accessioned2022-10-21
dc.date.available2023-10-09T22:52:23Z
dc.date.created2022
dc.date.issued2022-10-21
dc.description.abstractPreviously the effect of the pruritogens, such as histamine and chloroquine, was tested in 11 inbred mouse strains, and this study aimed to identify resistant and sensitive strains, consistent with the observation that underlies the large variability in human populations. In the present study, we used the low responder C3H/HeJ (C3H) and the more sensitive C57BL/6J (C57) strain to find out if resistance and sensitivity to develop pruritus is restricted to only histamine and chloroquine or extends to other known pruritogens as well. We tested five additional commonly known pruritogens. We established dose-response relationships by injecting four concentrations of the pruritogens in the range of 0.3, 1, 3, and ten-fold in the nuchal fold. Then we assessed the scratching behavior for 30 min after injection with an automated custom-designed device based on the bilateral implantation of mini-magnets in the hind paws and on single cages placed within a magnetic coil. We found that the resistance to pruritogens is a general phenotype of the C3H strain and extends to all pruritogens tested, including not only histamine and chloroquine, but also endothelin, trypsin, 5-HT (serotonin), the short peptide SLIGRL, and Lysophosphatidic acid (LPA). C57 was more sensitive to all pruritogens and, in contrast to C3H, dose-response relationships were evident for some of the pruritogens. In general, comparable peak scratch responses were observed for the 0.3-fold concentrations of the pruritogens in C57 whereas C3H required at least the ten-fold concentration and still displayed only between 5 and 33% of the scratch responses observed in C57 for the respective pruritogen. The general resistance to pruritogens and the low level of scratching behavior found in the C3H strain is an interesting trait and represents a model for the study of the heritability of itch. It is accompanied in C3H with a higher sensitivity in assays of nociception.en
dc.identifier.citationFrontiers in Molecular Neuroscience 15 (2022): 934564. <https://www.frontiersin.org/articles/10.3389/fnmol.2022.934564/full>
dc.identifier.doihttps://doi.org/10.3389/fnmol.2022.934564
dc.identifier.issn1662-5099
dc.identifier.opus-id20704
dc.identifier.urihttps://open.fau.de/handle/openfau/20704
dc.identifier.urnurn:nbn:de:bvb:29-opus4-207048
dc.language.isoen
dc.publisherFrontiers Media S.A.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjectpruritus
dc.subjectscratching behavior
dc.subjectitch
dc.subjectinbred mouse strains
dc.subjectC57BL/6J
dc.subjectheritability
dc.subject.ddcDDC Classification::6 Technik, Medizin, angewandte Wissenschaften :: 61 Medizin und Gesundheit :: 610 Medizin und Gesundheit
dc.titleGeneralized resistance to pruritogen-induced scratching in the C3H/HeJ strainen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.date.prevpublished2022-10-05
local.document.articlenumber934564
local.journal.titleFrontiers in Molecular Neuroscience
local.journal.volume15
local.sendToDnbfree*
local.subject.fakultaetMedizinische Fakultät
local.subject.importimport
local.subject.sammlungUniversität Erlangen-Nürnberg / Von der FAU geförderte Open Access Artikel / Von der FAU geförderte Open Access Artikel 2022
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