Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication

Language
en
Document Type
Article
Issue Date
2019-08-13
First published
2019-07-31
Issue Year
2019
Authors
Kahl, Melanie
Gertig, Markus
Hoyer, Phillipp
Friedrich, Oliver
Gilbert, Daniel F.
Editor
Publisher
Frontiers Media S.A.
Abstract

3D bioprinting has become a versatile and powerful method in tissue engineering and regenerative medicine and is increasingly adapted by other disciplines due to its tremendous potential beyond its typical applications. However, commercially available 3D bioprinting systems are typically expensive circumventing the broad implementation, including laboratories in low-resource settings. To address the limitations of conventional and commercially available technology, we developed a 3D bioprinter by modification of an off-the-shelf 3D desktop printer, that can be installed within a single day, is of handy size to fit into a standard laminar flow hood, customizable, ultra-low cost and thus, affordable to a broad range of research labs, or educational institutions. We evaluate accuracy and reproducibility of printing results using alginate and alginate/gelatin-hydrogels and demonstrate its potential for biomedical use by printing of various two-and three-dimensional cell-free and mammalian cell-laden objects using recombinant HEKYFP cells, stably expressing yellow fluorescent protein (YFP) as a model system and high-content imaging. We further provide a parts list and 3D design files in STL and STEP format for reconstructing the device. A time-lapse video of the custom-built device during operation is available at https://vimeo.com/274482794.

Journal Title
Frontiers in Bioengineering and Biotechnology
Volume
7
Citation

Frontiers in Bioengineering and Biotechnology 7 (2019). https://www.frontiersin.org/articles/10.3389/fbioe.2019.00184/full

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