Micro-Computed-Tomography-Guided Analysis of In Vitro Structural Modifications in Two Types of 45S5 Bioactive Glass Based Scaffolds

dc.contributor.authorWesthauser, Fabian
dc.contributor.authorCiraldo, Francesca
dc.contributor.authorBalasubramanian, Preethi
dc.contributor.authorSenger, Anne-Sophie
dc.contributor.authorSchmidmaier, Gerhard
dc.contributor.authorMoghaddam, Arash
dc.contributor.authorBoccaccini, Aldo
dc.date.accessioned2019-08-13
dc.date.available2023-10-16T21:35:02Z
dc.date.created2017
dc.date.issued2019-08-13
dc.description.abstractThree-dimensional 45S5 bioactive glass (BG)-based scaffolds are being investigated for bone regeneration. Besides structural properties, controlled time-dependent alteration of scaffold morphology is crucial to achieve optimal scaffold characteristics for successful bone repair. There is no in vitro evidence concerning the dependence between structural characteristics and dissolution behavior of 45S5 BG-based scaffolds of different morphology. In this study, the dissolution behavior of scaffolds fabricated by the foam replica method using polyurethane foam (Group A) and maritime sponge Spongia Agaricina (Group B) as sacrificial templates was analyzed by micro-computed-tomography (µCT). The scaffolds were immersed in Dulbecco’s Modified Eagle Medium for 56 days under static cell culture conditions and underwent µCT-analysis initially, and after 7, 14, and 56 days. Group A showed high porosity (91%) and trabecular structure formed by macro-pores (average diameter 692 µm ± 72 µm). Group-B-scaffolds were less porous (51%), revealing an optimal pore size distribution within the window of 110–500 µm pore size diameter, combined with superior mechanical stability. Both groups showed similar structural alteration upon immersion. Surface area and scaffold volume increased whilst density decreased, reflecting initial dissolution followed by hydroxycarbonate-apatite-layer-formation on the scaffold surfaces. In vitro- and/or in vivo-testing of cell-seeded BG-scaffolds used in this study should be performed to evaluate the BG-scaffolds’ time-dependent osteogenic properties in relation to the measured in vitro structural changes.en
dc.identifier.citationMaterials 10.12 (2017). <https://www.mdpi.com/1996-1944/10/12/1341>
dc.identifier.doihttps://doi.org/10.3390/ma10121341
dc.identifier.opus-id11384
dc.identifier.urihttps://open.fau.de/handle/openfau/11384
dc.identifier.urnurn:nbn:de:bvb:29-opus4-113849
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjectbioactive Glass
dc.subjectpolyurethane foam
dc.subjectmaritime national sponge
dc.subjectµCT
dc.subjecttissue engineering
dc.subjectin vitro
dc.subjectdissolution behavior
dc.subject.ddcDDC Classification::6 Technik, Medizin, angewandte Wissenschaften :: 60 Technik :: 600 Technik, Technologie
dc.titleMicro-Computed-Tomography-Guided Analysis of In Vitro Structural Modifications in Two Types of 45S5 Bioactive Glass Based Scaffoldsen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.date.prevpublished2017-11-23
local.journal.issue12
local.journal.titleMaterials
local.journal.volume10
local.sendToDnbfree*
local.subject.fakultaetTechnische Fakultät
local.subject.importimport
local.subject.sammlungUniversität Erlangen-Nürnberg / Eingespielte Open Access Artikel / Eingespielte Open Access Artikel 2019
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
11384_materials-10-01341.pdf
Size:
1.53 MB
Format:
Adobe Portable Document Format
Description: