Mitochondrial respiration in B lymphocytes is essential for humoral immunity by controlling the flux of the TCA cycle

Language
en
Document Type
Article
Issue Date
2023-06-29
First published
2022-06-07
Issue Year
2022
Authors
Urbanczyk, Sophia
Baris, Olivier R.
Hofmann, Jörg
Verena Taudte, R.
Guegen, Naïg
Golombek, Florian
Castiglione, Kathrin
Meng, Xianyi
Bozec, Aline
Thomas, Jana
Editor
Abstract

To elucidate the function of oxidative phosphorylation (OxPhos) during B cell differentiation, we employ CD23Cre-driven expression of the dominant-negative K320E mutant of the mitochondrial helicase Twinkle (DNT). DNT-expression depletes mitochondrial DNA during B cell maturation, reduces the abundance of respiratory chain protein subunits encoded by mitochondrial DNA, and, consequently, respiratory chain super-complexes in activated B cells. Whereas B cell development in DNT mice is normal, B cell proliferation, germinal centers, class switch to IgG, plasma cell maturation, and T cell-dependent as well as T cell-independent humoral immunity are diminished. DNT expression dampens OxPhos but increases glycolysis in lipopolysaccharide and B cell receptor-activated cells. Lipopolysaccharide-activated DNT-B cells exhibit altered metabolites of glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle and a lower amount of phosphatidic acid. Consequently, mTORC1 activity and BLIMP1 induction are curtailed, whereas HIF1α is stabilized. Hence, mitochondrial DNA controls the metabolism of activated B cells via OxPhos to foster humoral immunity.

Journal Title
Cell Reports
Volume
39
Issue
10
Citation
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