Validation of the Slow Off‐Kinetics of Sirtuin‐Rearranging Ligands (SirReals) by Means of Label‐Free Electrically Switchable Nanolever Technology†

Language
en
Document Type
Article
Issue Date
2020-10-01
First published
2020-04-24
Issue Year
2020
Authors
Schiedel, Matthias
Daub, Herwin
Itzen, Aymelt
Jung, Manfred
Editor
Abstract

We have discovered the sirtuin‐rearranging ligands (SirReals) to be highly potent and selective inhibitors of the NAD+‐dependent lysine deacetylase Sirt2. Using a biotinylated SirReal in combination with biolayer interferometry, we previously observed a slow dissociation rate of the inhibitor–enzyme complex; this had been postulated to be the key to the high affinity and selectivity of SirReals. However, to attach biotin to the SirReal core, we introduced a triazole as a linking moiety; this was shown by X‐ray co‐crystallography to interact with Arg97 of the cofactor binding loop. Herein, we aim to elucidate whether the observed long residence time of the SirReals is induced mainly by triazole incorporation or is an inherent characteristic of the SirReal inhibitor core. We used the novel label‐free switchSENSE® technology, which is based on electrically switchable DNA nanolevers, to prove that the long residence time of the SirReals is indeed caused by the core scaffold.

Journal Title
ChemBioChem
Volume
21
Issue
8
Citation
ChemBioChem 21.8 (2020): 1161-1166. <https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cbic.201900527>
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