Selective retina therapy and thermal stimulation of the retina: different regenerative properties - implications for AMD therapy

dc.contributor.authorRichert, Elisabeth
dc.contributor.authorPapenkort, Julia
dc.contributor.authorBurchard, Claus von der
dc.contributor.authorKlettner, Alexa
dc.contributor.authorArnold, Philipp
dc.contributor.authorLucius, Ralph
dc.contributor.authorBrinkmann, Ralf
dc.contributor.authorFramme, Carsten
dc.contributor.authorRoider, Johann
dc.contributor.authorTode, Jan
dc.date.accessioned2021-12-17
dc.date.available2021-12-16
dc.date.created2021
dc.date.issued2021-12-17
dc.description.abstractBackground Selective Retina Therapy (SRT), a photodisruptive micropulsed laser modality that selectively destroys RPE cells followed by regeneration, and Thermal Stimulation of the Retina (TSR), a stimulative photothermal continuous wave laser modality that leads to an instant sublethal temperature increase in RPE cells, have shown therapeutic effects on Age-related Macular Degeneration (AMD) in mice. We investigate the differences between both laser modalities concerning RPE regeneration. Methods For PCR array, 6 eyes of murine AMD models, apolipoprotein E and nuclear factor erythroid-derived 2- like 2 knock out mice respectively, were treated by neuroretina-sparing TSR or SRT. Untreated litter mates were controls. Eyes were enucleated either 1 or 7 days after laser treatment. For morphological analysis, porcine RPE/choroid organ cultures underwent the same laser treatment and were examined by calcein vitality staining 1 h and 1, 3 or 5 days after irradiation. Results TSR did not induce the expression of cell-mediators connected to cell death. SRT induced necrosis associated cytokines as well as inflammation 1 but not 7 days after treatment. Morphologically, 1 h after TSR, there was no cell damage. One and 3 days after TSR, dense chromatin and cell destruction of single cells was seen. Five days after TSR, there were signs of migration and proliferation. In contrast, 1 h after SRT a defined necrotic area within the laser spot was seen. This lesion was closed over days by migration and proliferation of adjacent cells. Conclusions SRT induces RPE cell death, followed by regeneration within a few days. It is accompanied by necrosis induced inflammation, RPE proliferation and migration. TSR does not induce immediate RPE cell death; however, migration and mitosis can be seen a few days after laser irradiation, not accompanied by necrosis-associated inflammation. Both might be a therapeutic option for the treatment of AMD.en
dc.identifier.citationBMC Ophthalmology 21 (2021): 512. <https://bmcophthalmol.biomedcentral.com/articles/10.1186/s12886-021-02188-8>
dc.identifier.doihttps://doi.org/10.1186/s12886-021-02188-8
dc.identifier.opus-id17930
dc.identifier.urihttps://open.fau.de/handle/openfau/17930
dc.identifier.urnurn:nbn:de:bvb:29-opus4-179305
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjectSelective retina therapy (SRT)
dc.subjectThermal stimulation of the retina (TSR)
dc.subjectAge- related macular degeneration (AMD)
dc.subjectRegeneration
dc.subjectRejuvenation
dc.subject.ddcDDC Classification::6 Technik, Medizin, angewandte Wissenschaften :: 61 Medizin und Gesundheit :: 610 Medizin und Gesundheit
dc.titleSelective retina therapy and thermal stimulation of the retina: different regenerative properties - implications for AMD therapyen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.document.articlenumber412
local.journal.titleBMC Ophthalmology
local.journal.volume21
local.sendToDnbfree*
local.subject.fakultaetMedizinische Fakultät
local.subject.sammlungUniversität Erlangen-Nürnberg / Open Access Artikel ohne Förderung / Open Access Artikel ohne Förderung 2021
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