A caspase–RhoGEF axis contributes to the cell size threshold for apoptotic death in developing Caenorhabditis elegans

dc.contributor.authorSethi, Aditya
dc.contributor.authorWei, Hai
dc.contributor.authorMishra, Nikhil
dc.contributor.authorSegos, Ioannis
dc.contributor.authorLambie, Eric J.
dc.contributor.authorZanin, Esther
dc.contributor.authorConradt, Barbara
dc.date.accessioned2023-01-24
dc.date.available2023-10-11T13:20:57Z
dc.date.created2022
dc.date.issued2023-01-24
dc.description.abstractA cell’s size affects the likelihood that it will die. But how is cell size controlled in this context and how does cell size impact commitment to the cell death fate? We present evidence that the caspase CED-3 interacts with the RhoGEF ECT-2 in Caenorhabditis elegans neuroblasts that generate “unwanted” cells. We propose that this interaction promotes polar actomyosin contractility, which leads to unequal neuroblast division and the generation of a daughter cell that is below the critical “lethal” size threshold. Furthermore, we find that hyperactivation of ECT-2 RhoGEF reduces the sizes of unwanted cells. Importantly, this suppresses the “cell death abnormal” phenotype caused by the partial loss of ced-3 caspase and therefore increases the likelihood that unwanted cells die. A putative null mutation of ced-3 caspase, however, is not suppressed, which indicates that cell size affects CED-3 caspase activation and/or activity. Therefore, we have uncovered novel sequential and reciprocal interactions between the apoptosis pathway and cell size that impact a cell’s commitment to the cell death fate.en
dc.identifier.citationPLoS Biology 20.10 (2022): e3001786. <https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001786>
dc.identifier.doihttps://doi.org/10.1371/journal.pbio.3001786
dc.identifier.opus-id21383
dc.identifier.urihttps://open.fau.de/handle/openfau/21383
dc.identifier.urnurn:nbn:de:bvb:29-opus4-213833
dc.language.isoen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.de
dc.subjectApoptosis
dc.subjectCell cycle and cell division
dc.subjectNeuroblasts
dc.subjectCaenorhabditis elegans
dc.subjectMetaphase
dc.subjectMyosins
dc.subjectCell membranes
dc.subjectLarvae
dc.subject.ddcDDC Classification::5 Naturwissenschaften und Mathematik :: 57 Biowissenschaften; Biologie :: 570 Biowissenschaften; Biologie
dc.titleA caspase–RhoGEF axis contributes to the cell size threshold for apoptotic death in developing Caenorhabditis elegansen
dc.typearticle
dcterms.publisherFriedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
local.date.prevpublished2022-10-06
local.document.articlenumbere3001786
local.journal.issue10
local.journal.titlePLoS Biology
local.journal.volume20
local.sendToDnbfree*
local.subject.fakultaetNaturwissenschaftliche Fakultät
local.subject.sammlungUniversität Erlangen-Nürnberg / Open Access Artikel ohne Förderung / Open Access Artikel ohne Förderung 2023
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
21383_journal.pbio.3001786.pdf
Size:
2.4 MB
Format:
Adobe Portable Document Format
Description: