TRPC6 Is Found in Distinct Compartments of the Human Kidney

Language
en
Document Type
Article
Issue Date
2022-10-21
First published
2022-04-24
Issue Year
2022
Authors
Englisch, Colya N.
Röhricht, Daniel
Walz, Mariesa
Junker, Kerstin
Beckmann, Anja
Meier, Carola
Paulsen, Friedrich
Jung, Martin
Tschernig, Thomas
Editor
Publisher
MDPI
Abstract

In the context of renal proteinuric diseases, TRPC6 has been shown to play an important role in ultrafiltration associated with the slit diaphragm through the control of the intracellular Ca2+ concentration in the podocytes of glomeruli. However, to date, the properties of TRPC6 have been studied mainly in cell lines or in animal models. Therefore, the aim of the study presented here was to investigate the presence and distribution of TRPC6 in human kidneys in order to possibly verify the applicability of the results previously obtained in nonhuman experiments. For this purpose, kidneys from nine cadavers were prepared for immunohistochemical staining and were supplemented with a fresh human kidney obtained by nephrectomy. TRPC6 was detected in glomeruli and in the parietal epithelial cells of Bowman’s capsule. Larger amounts were detected in the tubular system and collecting ducts. In contrast to the peritubular capillary bed, which showed no immune reaction, the cortical resistance vessels showed mild TRPC6 staining. In conclusion, our studies on the expression of TRPC6 in human kidney tissue support the translational concept of the involvement of TRPC6 in various renal diseases and reveal new aspects of the distribution of TRPC6 in the human kidney.

Journal Title
International Journal of Translational Medicine
Volume
2
Issue
2
Citation
International Journal of Translational Medicine 2.2 (2022): S. 156-163. <https://www.mdpi.com/2673-8937/2/2/13>
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